A Simple and Economical Modification of the Masson-Fontana Method for Staining Melanin Granules and Enterochromaffin Cells
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 59 (4) , 193-196
- https://doi.org/10.3109/10520298409113855
Abstract
Enterochromaffin cells from the small intestine of man, guinea pig, dog, chicken, rabbit, cat and rat were stained using the Masson-Fontana ammonical silver method with varying dilutions of silver nitrate solution (0.25 to 5 g per 100 ml of distilled water) and incubation temperatures (60.degree. and 75.degree. C). The 0.5% solution of silver nitrate gave an argentaffin pattern similar to that of the 5% solution and had 2 major advantages: economically, since much less silver nitrate is used, and methodologically, since low background resulted with tissue of those species (rat, cat and rabbit) that required unusually long incubation. The staining of melanocytes was similar for all dilutions at the usual staining time (15-30 min).This publication has 1 reference indexed in Scilit:
- Differential staining of catecholamines, 5-hydroxytryptamine and related compounds in aldehyde-fixed tissuesHistochemistry and Cell Biology, 1969