Sequence organization of the circular plasmid pKDl from the yeastKluyveromyces drosophilarum
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (11) , 4471-4481
- https://doi.org/10.1093/nar/14.11.4471
Abstract
PKDl is the only circular plasmid known in the genus Kluyveromyces. Nucleotide sequence analysis has revealed that this 4757 base-pairs long plasmid contained three major open reading frames, A, B, and C, and a pair of inverted repeats of 346 base-pairs. The molecule exists in two isomeric forms generated by internal recombination at these repeats. The functional organization of pKDl genome appears to be quite analogous to that of the 2u plasmid of Saccharomyces cerevisiae. There is however little homology of sequences between these plasmids, except that the gene A has a dispersed but significant homology with the FLP recombinase gene of the 2u plasmid. S.cerevisiae cells can be transformed by derivatives of pKDl carrying URA3 gene as a selection marker.Keywords
This publication has 6 references indexed in Scilit:
- Molecular and functional organization of yeast plasmid pSR1Journal of Molecular Biology, 1985
- Physical and functional structure of a yeast plasmid, pSB3, isolated fromZygosaccharomyces bisporusNucleic Acids Research, 1985
- [18] Hybridization of denatured RNA transferred or dotted to nitrocellulose paperPublished by Elsevier ,1983
- Nucleotide sequence of the yeast plasmidNature, 1980
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- Spliced early mRNAs of simian virus 40Proceedings of the National Academy of Sciences, 1978