Effect of prolonged staining with hematoxylin on detectingHelicobacter pyiori inhematoxylin‐eosin‐stained gastric mucosa

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Abstract
Helicohacter pylorl (H. pylorl) Is ldentlflable in hematoxyiin‐eosin (HE)‐stalned preparations, particuularly under a prolonged hematoxylln staining condition. The stalnlng intensity ofH. pyloribodies was Increased when the hemetoxylln stalnlng period was doubled from 5 min, the routine period of Meyer's hematoxyiin stalnlng, to 10 min. The stalning intenstty of the background eplthellal nuclei was only mildly increased. Hematoxylin‐eosin staining employing a prolonged hematoxylin staining period gave detedablllty ofH. pyloricomparable to May‐Glemsa and lmmunoperoxldase staining. The modified HE method is thus Very useful and practical for identifyingH. pyloriin routine gastric biopsy specimens.