Transcription complexes that program Xenopus 5S RNA genes are stable in vivo.
- 1 August 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (15) , 5516-5520
- https://doi.org/10.1073/pnas.85.15.5516
Abstract
The long-term stability of transcription complexes on 5S RNA genes has been demonstrated in vivo. Complexes on oocyte and somatic-type 5S RNA genes injected into Xenopus laevis oocytes nuclei are stable for at least 4 days. Tissue culture cells and mature erythrocytes have equivalent numbers of somatic 5S RNA genes programed into transcription complexes, yet the former cell type has a > 50-fold higher cellular content of transcription factor IIIA (TFIIIA). Functional transcription complexes on somatic 5S RNA genes in nucleated erythrocytes of Xenopus are stable for weeks, perhaps months, even though a mature erythrocyte has less than two molecules of TFIIIA for each somatic 5S RNA gene. These findings strengthen our proposal that stable transcription complexes are a means of maintaining the differentiated state.Keywords
This publication has 35 references indexed in Scilit:
- Differential 5S RNA gene expression in vitroCell, 1987
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- The yeast mitochondrial ATPase complex. Subunit composition and evidence for a latent protease contaminantBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1979
- The transcription of 5 S DNA injected into Xenopus oocytesDevelopmental Biology, 1978
- A nuclear extract of xenopus laevis oocytes that accurately transcribes 5S RNA genesCell, 1978
- Template-engaged and free RNA polymerases during Xenopus erythroid cell maturationDevelopmental Biology, 1978
- Sequence organization of a cloned tDNA1met fragment from xenopus laevisCell, 1978
- High-fidelity transcription of 5S DNA injected into Xenopus oocytes.Proceedings of the National Academy of Sciences, 1977
- Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.Proceedings of the National Academy of Sciences, 1977
- Protein incorporation by isolated amphibian oocytes III. Optimum incubation conditionsJournal of Experimental Zoology, 1973