Role of locally formed angiotensin II and bradykinin in the reduction of myocardial infarct size in dogs

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Abstract
Objective: The aim was to investigate the role of local formation of angiotensin II and bradykinin in the reduction of myocardial infarct size. Methods: Bilaterally nephrectomised male mongrel dogs were used. Effects were compared of pretreatment with three inhibitors of angiotensin II forming enzyme – captopril (an angiotensin converting enzyme inhibitor), nafamostat (a serine protease inhibitor), and chymostatin (a cysteine protease inhibitor) - on left anterior descending coronary artery occlusion. Haemodynamic variables were monitored and blood was collected from the anterior interventricular vein and the aorta. Angiotensin I, angiotensin II, and bradykinin were measured by radioimmunoassay. After 90 min of occlusion, infarct sizes were determined by a macroscopic enzyme technique. Results: Angiotensin II release into the anterior interventricular vein increased from 0.03(SEM 1.19) pg·min−1 (before coronary occlusion) to 4.64(1.37) pg·min−1 (n=14, p−1 (n=14 p−1 at 5 min after occlusion (n=12, pConclusions: Angiotensin II is locally produced in the ischaemic heart by both serine protease(s) and chymostatin inhibitable protease(s), but not by angiotensin converting enzyme. From the reduction in myocardial infarct size produced by angiotensin converting enzyme inhibition, it seems that bradykinin accumulation may play a more important role than the suppression of angiotensin II formation. Cardiovascular Research 1993;27:334-340

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