ANTI-DNP ANTIBODY-RESPONSE AFTER TOPICAL APPLICATION OF DNFB IN MICE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 33  (4) , 589-596
Abstract
A single painting or daily paintings for 5 days with dinitrofluorobenzene (DNFB) on the abdominal skin of mice induced both contact sensitivity, detectable by ear swelling, and, hapten-reactive helper T [thymus-derived] cells, detectable by the augmented anti-bovine serum albumin (BSA) antibody response on challenge with dinitrophenyl-BSA. Contact sensitivity was induced within 7 days and helper activity within 14 days after the sensitization. Anti-hapten antibody response in the spleen or regional lymph nodes of such mice was negligibly small during the 15 days after a single painting. Failure to respond with anti-hapten antibody production of mice given only a single painting was due to the shortage of B [bone marrow-derived] cells reactive to the hapten. Daily paintings for 5 days did not necessarily result in the augmented antibody response. A strong anti-hapten antibody response was observed in mice receiving 2 paintings at an interval of 10 days. In these mice, hapten-specific B memory cells as well as hapten-reactive T cells were detected. The anti-hapten antibody response after the topical application of simple chemicals may depend upon the priming of B cells, and the response must be mediated by the cooperation of T and B cells both reactive to the antigen.