Fine Structure and Biomineralization of the Mucilage in Envelopes ofTrachelomonas lefevrei(Euglenophyceae)1

Publisher Website
Google Scholar
Abstract
Envelope development inTrachelomonas lefevrei(Deflandre) begins with the production of short, coarse mucilaginous strands, morphologically similar to the mucilage produced by non‐enveloped euglenoids. These initial mucilaginous secretions subsequently become impregnated with manganese (Mn) and/or iron (Fe). Continued mucilage secretion and mineralization results in a mature envelope that is characteristic for the species. When these mature envelopes are treated with oxalic acid to remove the Mn and Fe, the envelopes collapse and are composed only of short, coarse mucilaginous strands similar to those present during early stages of development, prior to their mineralization. Brief treatment with 10 mM EDTA renders dark envelopes colorless, and our EM‐EDS analyses show that this corresponds to a loss of Mn from the envelope; however, if Fe is present in the envelope, it is unaffected by the brief treatment. The mucilage present during early stages of envelope development and that remaining after complete demineralization is also morphologically similar to the mucilage in the plug at the anterior end of the envelope.