Folic acid compounds in romaine lettuce
- 1 August 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 55 (8) , 865-868
- https://doi.org/10.1139/o77-127
Abstract
The composition of folate coenzymes in romaine lettuce was studied. Lettuce extract was purified on QAE[quaternary ammonium ethyl]-Sephadex A-25 and folate compounds were separated into a monoglutamate fraction and a polyglutamate fraction by chromatography on Sephadex G-15. Both the mono- and poly-glutamate fractions were resolved on DEAE-cellulose. Positive identification of DEAE peaks was made by further cochromatography with high specific activity radioactive marker folate compounds and with differential microbiological [pteroylglutamic acid] [Lactobacillus casei and Streptococcus faecalis] assay. The distribution of folate compounds in lettuce is as follows: 32% 5-CH3-H4PteGlu; 1% 5-CHO-H4PteGlu; 3% 5-CHO-H4PteGlu4; 9% 5-CH3-H4PteGlu4; 13% 5-CHO-H4PteGlu5; and 31% 5-CH3-H4PteGlu5.This publication has 1 reference indexed in Scilit:
- Studies on the folic acid activity of human serumJournal of Clinical Pathology, 1961