Altered substrate selectivity of PKC‐η pseudosubstrate site mutants
- 23 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 329 (1-2) , 129-133
- https://doi.org/10.1016/0014-5793(93)80208-c
Abstract
Three protein kinase C (PKC)‐η mutants were constructed in which the whole or part of the pseudosubstrate site was replaced with corresponding parts of the PKC‐α pseudosubstrate site. The resulting chimaeric kinases were compared with wild‐type PKC‐η in their ability to phosphorylate a PKC‐η peptide substrate or histone. Changes in the pseudosubstrate site of pkc‐η are accompanied by changes in substrate selectivity, indicating that the substrate selectivity observed for PKC‐η is at least in part due to its pseudosubstrate site.Keywords
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