Fine structural localization of periodic acid-thio-carbohydrazide-silver proteinate reactive substances in rat thyroid follicular cells.

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Abstract
Rat thyroid gland tissues, fixed in various fixatives, were stained by the periodic acid thiocarbohydrazide silver proteinate (PA-TCH-SP) method of Thiery and observed at the ultrastructural level. In EM observations, the cell organelles showing positive reactions were the Golgi apparatus, subapical granules, reabsorbed colloid droplets, lysosomes of follicular epithelial cells and colloid of the follicular lumen. The effect of fixatives was compared with respect to the differences in stainability. In each tissue fixed either with glutaraldehyde and OsO4 or OsO4 alone only a trace of the Ag reaction product was discernible. A 1 h treatment of TCH was insufficient for tissues fixed singly with paraformaldehyde or glutaraldehyde, and the stainability was strongly intensified by prolongation of TCH immersion, especially in tissues fixed only with paraformaldehyde. To intensify the PA-TCH-SP reaction, protonation with acetic acid and etching using KOH were performed on the sections fixed singly in OsO4 or glutaraldehyde, respectively. Favorable results were obtained from each treatment. Although the PA-TCH-SP method is analogous to the PAS [periodic acid Schiff] reaction in principle, the reaction is probably influenced by the kind of fixative and the amount of reactive sugar.

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