3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthase from Potato Tuber (Solanum tuberosum L.)
Open Access
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 82 (4) , 1040-1044
- https://doi.org/10.1104/pp.82.4.1040
Abstract
3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway, was purified to electrophoretic homogeneity from tubers of Solanum tuberosum L. cv Superior. The enzyme is a dimer with a native molecular weight of 110,000. The enzyme appears to be hysteretic. The enzyme activity is stimulated by Mn2+ and l-tryptophan. Chromatofocusing resolved two forms of the enzyme with isoelectric points of 7.8 and 8.4, respectively. The enzyme closely resembles an analogous activity previously isolated from roots of Daucus carota (JA Suzich, JFD Dean, KM Herrmann 1985 Plant Physiol 79: 765-770).This publication has 17 references indexed in Scilit:
- Differentially Regulated Isozymes of 3-Deoxy-d-arabino-Heptulosonate-7-Phosphate Synthase from Seedlings of Vigna radiata [L.] WilczekPlant Physiology, 1985
- 3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthase from Carrot Root (Daucus carota) Is a Hysteretic EnzymePlant Physiology, 1985
- Regulation of the Shikimate Pathway of Carrot Cells in Suspension CulturePlant Physiology, 1984
- Shikimate Pathway Activity during Shoot Initiation in Tobacco Callus CulturesPlant Physiology, 1983
- Biosynthesis of aromatic compounds: 13C NMR spectroscopy of whole Escherichia coli cells.Proceedings of the National Academy of Sciences, 1982
- Enzymological Basis for Herbicidal Action of GlyphosatePlant Physiology, 1982
- 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification and molecular characterization of the phenylalanine-sensitive isoenzyme from Escherichia coli.Journal of Biological Chemistry, 1978
- 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli.Journal of Biological Chemistry, 1976
- 2-Keto-3-deoxy-d-arabo-heptonic Acid 7-Phosphate SynthetaseJournal of Biological Chemistry, 1959
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951