Induction of Apoptosis by Coprinus disseminatus Mycelial Culture Broth Extract in Human Cervical Carcinoma Cells.
- 1 January 1999
- journal article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 24 (4) , 209-215
- https://doi.org/10.1247/csf.24.209
Abstract
Extract of Coprinus disseminatus (pers. Fr.) (C. disseminatus) culture broth (EDCB) inhibits proliferation and induces apoptosis in the human cervical carcinoma cells at 5 microg/ml. To determine whether the cell death induced by the EDCB recruits caspases or not, one of the exclusive pathways in cell death, we examined caspase-3 activity in this cell death process. The activity of caspase-3 was remarkably increased when the cell was treated with EDCB, and this activity was nullified by Z-VAD-FMK, a well known caspase-3 inhibitor. From these results, we would expect the EDCB to contain substances with the ability to induce apoptosis in the human cervical carcinoma cells. The extent of the EDCB induced apoptosis is cell line-dependent.Keywords
This publication has 18 references indexed in Scilit:
- Excitotoxins in Neuronal Apoptosis and NecrosisJournal of Cerebral Blood Flow & Metabolism, 1999
- FLICE Induced Apoptosis in a Cell-free SystemJournal of Biological Chemistry, 1997
- Apoptosis: Molecular Regulation of Cell DeathEuropean Journal of Biochemistry, 1996
- Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosisNature, 1995
- Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3Cell, 1993
- The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzymeCell, 1993
- Directly acting geno- and cytotoxic agents from a wild mushroom Dermocybe sanguineaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1992
- Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assaysJournal of Immunological Methods, 1983
- Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activationNature, 1980