Isolation and Characterization of an Iron-Containing Superoxide Dismutase From Water Lily, Nuphar luteum

Abstract
A cyanide-insensitive superoxide dismutase (EC 1.15.1.1) was purified from leaves of the water lily N. luteum (L.) Sibth. and Smith ssp. macrophyllum (Small) Beal. The enzyme has a MW of 46,000 and is composed of 2 equally sized subunits. Metal analysis showed that the protein contains .apprx. 1 gram atom of Fe per dimer. The Fe-containing superoxide dismutase was sensitive to H2O2 as well as to azide. Antibody to the protein did not cross-react with Fe superoxide dismutase isolated from the eucaryote Brassica or with algal extracts.