Analysis for geometrical and positional isomers of fatty acids in partially hydrogenated fats

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Abstract
A liquid Chromatographic procedure for frac‐tionation and analysis of methyl esters from fats is described. First, esters are separated by liquid chromatography on a partially vulcanized rubber column into trienoate, dienoate, monoenoate‐palmitate and stearate fractions. The monoenoate‐palmitate fraction is separated by liquid chro‐matography on a silver‐saturated cation exchange resin into palmitate,trans monoenoate and cis monoenoate fractions. Double bond positions intrans and cis monoenoates are located by ozoniza‐tion and gas chromatography of fragments. This separation procedure requires less time and uses simpler apparatus with smaller samples than a previously described method based on counter‐current distribution. Data from analyses of two liquid oils and four shortenings are presented.