Analysis of deoxynivalenol from cultures of Fusarium species

Abstract
Eight isolates of Fusarium Roseum and 3 of F. culmorum were found to produce deoxynivalenol in rice cultures. Deoxynivalenol was extracted with aqueous methanol (40%) and purified by partitioning with ethyl acetate and acetonitrile-petroleum ether (boiling point, 60-70.degree. C). The toxin was identified by gas chromatography/mass spectrometry and quantified by GLC. High recoveries (80%) of deoxynivalenol were obtained from rice cultures, and as low as 0.250 .mu.g of the toxin per gram was detected.

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