A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli

15 May 1991

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 80 (2-3) , 147-150
https://doi.org/10.1016/0378-1097(91)90585-x

Abstract

Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Ap^r), gentamicin (Gm^r) and amikacin (Ak^r) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 10³ transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure.

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