One-tube post-PCR fluorescent labeling of DNA fragments.
Open Access
- 1 June 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 6 (6) , 551-557
- https://doi.org/10.1101/gr.6.6.551
Abstract
A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis.Keywords
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