Recognition of prokaryotic transcription terminators by spinach chloroplast RNA polymerase
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (17) , 8411-8431
- https://doi.org/10.1093/nar/16.17.8411
Abstract
To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro we have fused the spinach chloroplast rbcL promoter to the 3'' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, rrnB, rrnC and gene 32 terminators. The most efficient transcription termination was observed for the threonine attenuator. For each of the prokaryotic terminators, the chloroplast enzyme ceased transcription at essentially the same position as the E. coli RNA polymerase. These data indicate that the transcription termination process in chloroplasts has some features in common with the mechanism used to prokaryotes.Keywords
This publication has 33 references indexed in Scilit:
- Gene organization and primary structure of a ribosomal RNA operon from Escherichia coliPublished by Elsevier ,2004
- Control of plastid gene expression: 3′ inverted repeats act as mRNA processing and stabilizing elements, but do not terminate transcriptionCell, 1987
- nusA Protein of Escherichia coli is an efficient transcription termination factor for certain terminator sitesJournal of Molecular Biology, 1987
- TRANSCRIPTION TERMINATION AND THE REGULATION OF GENE EXPRESSIONAnnual Review of Biochemistry, 1986
- Spinach plastid genes coding for initiation factor IF-1, ribosomal protein S11 and RNA polymerase -subunitNucleic Acids Research, 1986
- In vitro transcription of chloroplast protein genesPublished by Elsevier ,1986
- Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineeringProtein Engineering, Design and Selection, 1986
- In vitro insertional mutagenesis with a selectable DNA fragmentGene, 1984
- Structures of the genes for the β and ε subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signalProceedings of the National Academy of Sciences, 1982
- Nucleotide sequences involved in bacteriophage T4 gene 32 translational self-regulation.Proceedings of the National Academy of Sciences, 1982