Cannulation Without Visual Control of Minute Mesenteric Veins for Continuous Portal Perfusion of Rats Intoxicated With Bromobenzene

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Abstract
A special needle is used for the cannulation of minute mesenteric veins in the rat; it permits the introduction of a plastic cannula into the venule without visual control. The continuous portal perfusion with a glucose, calcium, vitamins C and P solution of rats intoxicated with bromobenzene does not increase their survival rate; however, there is less edema, vacuolation and necrosis around the terminal hepatic venules in the rats perfused with the vitamin mixture. The in vivo microscopic observation of the transilluminated liver showed sinusoidal and venular constriction followed by edema of the vascular walls, 20 minutes after the subcutaneous or subperitoneal injection of bromobenzene (2 mg/g body weight). Injection of half this dose into the splenic pulp produced thick "sludge" in the sinusoids which could be broken up during the early phase by heparin.