Stoichiometry of K+/H+ antiport helps to explain extracellular pH 11 in a model epithelium
- 7 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 361 (2-3) , 153-156
- https://doi.org/10.1016/0014-5793(95)00146-z
Abstract
The stoichiometry of K+/H+ antiport was measured fluorometrically by the static head method in highly purified vesicles from goblet cell apical membranes of larval lepidopteran midgut. The measured stoichiometry of 1 K+/2 H+ explains how the antiport results in electrophoretic exchange of extracellular H+ for intracellular K+, driven by the voltage component of the proton-motive force of an H+ translocating V-ATPase that is located in the same membrane. In turn, the exchange of K+ for H+ helps to explain how the midgut contents are alkalinized to a pH of 11.Keywords
This publication has 22 references indexed in Scilit:
- Energy Transduction in Tonoplast Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue: H+/Substrate Stoichiometries for the H+-ATPase and H+-PPaseArchives of Biochemistry and Biophysics, 1993
- [40] Isolation of goblet cell apical membrane from tobacco hornworm midgut and purification of its vacuolar-type ATPasePublished by Elsevier ,1990
- Role of Midgut Electrogenic K+ Pump Potential Difference in Regulating Lumen K+ and Ph in Larval LepidopteraJournal of Experimental Biology, 1988
- Cation-stimulated ATPase activity in purified plasma membranes from tobacco hornworm midgutBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Isolation of separate apical, lateral and basal plasma membrane from cells of an insect epithelium. A procedure based on tissue organization and ultrastructureTissue and Cell, 1983
- Stoichiometric studies of the renal outer cortical brush border membraned-glucose transporterThe Journal of Membrane Biology, 1982
- Intracellular K+ activities and cell membrane potentials in a K+-transporting epithelium, the midgut of tobacco hornoworm (Manduca sexta)The Journal of Membrane Biology, 1982
- Adaptive Significance of Midgut pH in Larval LepidopteraThe American Naturalist, 1980
- Techniques for Rearing Laboratory Colonies of Tobacco Hornworms and Pink Bollworms1Annals of the Entomological Society of America, 1976
- The inhibition of enzymes by tanninsPhytochemistry, 1965