Catabolism of plasma albumin by the perfused rat liver

Publisher Website
Google Scholar
Abstract
The catabolism of serum albumin labeled with I131 was investigated in the isolated perfused rat-liver. A period of about 20 minutes intervenes between the introduction of labeled rat albumin into the perfusion circuit and the appearance of nonprotein-bound I131. A similar latent period is observed with heat-denatured bovine serum albumin. Iodinated protein solutions were "screened" by injection into rats for various periods before use in liver-perfusion studies, in order to remove the small proportion of molecules which are catabolized rapidly by the liver. With screening periods of up to 24 hours the subsequent rate of catabolism shows wide variation. Labeled albumin separated either by chromatography or electrophoresis and screened for 48 or 72 hours is broken down during 4 or 5 hours perfusion at a constant rate which corresponds to about 14% of the total albumin breakdown in vivo. The perfused liver is able to break down heat-denatured bovine serum albumin at 2-5 times the rate observed for screened rat albumin.

This publication has 12 references indexed in Scilit: