Quantitative assessment of Na+,K+-ATPase localization by direct and indirect p-nitrophenyl phosphatase methods.
Open Access
- 1 April 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (4) , 507-513
- https://doi.org/10.1177/35.4.3029215
Abstract
Na+,K+-ATPase histochemistry, using direct (Pb) and indirect (Mg/Sr-Co) p-nitrophenyl phosphatase methods, was assessed by scanning integrative microdensitometry of three classes of tubules in mouse and guinea pig kidneys. The methods yielded similar and appropriate patterns of activity distribution and inhibitor response. The indirect method gave preferable results, in that non-enzymic background was lower and rate of reaction product accumulation was considerably higher.This publication has 8 references indexed in Scilit:
- Histochemical localization of phosphatases in the pig placenta: II. Potassium-dependent and potassium-independent p-nitrophenyl phosphatases at high pH; relation to sodium-potassium-dependent adenosine triphosphatasePlacenta, 1986
- Histochemical localization of phosphotases in the pig placenta: I. Non-specific phosphatases and their relation to uteroferrinPlacenta, 1986
- Purification of mouse brain (Na+ + K+)-ATPase catalytic unit, characterization of antiserum, and immunocytochemical localization in cerebellum, choroid plexus, and kidney.Journal of Histochemistry & Cytochemistry, 1984
- Ultrastructural localization of Na+,K+-ATPase in rat and rabbit kidney medulla.The Journal of cell biology, 1981
- The use of a hidden metal-capture reagent for the measurement of Na+?K+-ATPase activity: A new concept in cytochemistryHistochemistry and Cell Biology, 1981
- A new one-step method for the cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activityHistochemistry and Cell Biology, 1980
- Ouabain binding to renal tubules of the rabbit.The Journal of cell biology, 1978
- The influence of some cations on an adenosine triphosphatase from peripheral nervesBiochimica et Biophysica Acta, 1957