Inhibition of Ureolysis in Rat Intestine with Some Antimicrobial Agents in Vivo and in Vitro

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Abstract
The effects of oral administration of 7 antibiotics and 6 chemotherapeutic agents on ureolysis were investigated in rats by measuring 14CO2 expired in breathing air after oral administration of 14C-urea (in vivo) and by determining urease activity ileal-cecal contents of treated rats (in vitro). Urease activity was not directly inhibited by these drugs. When antibiotics were administered orally, ureolysis was strongly decreased by clindamycin, followed by cefazolin, thiophenicol and neomycin (depression percentages in vivo and in vitro were 50-70% and 56-96%, respectively). The antibiotic mixture completely depressed ureolysis in vivo and in vitro. The antibiotics used inhibited growth of urease-positive bacteria in the intestinal flora of rats. The effects depended on their antibacterial characteristics. When sulfanilamide or nitrofuran derivatives were administered, ureolysis in vivo and in vitro was not depressed but activated. Metronidazol administration depressed ureolysis in some rats and not in others, possibly due to a difference in the intestinal flora of several urease-positive bacteria. Berberine, lactulose and pectic acid administration did not affect ureolysis.

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