THE EFFECT OF ULTRAVIOLET LIGHT ON ARRESTED HUMAN DIPLOID CELL POPULATIONS

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Abstract
Abstract— The results of experiments to determine an effect of UV (254 nm) on human diploid fibro‐blasts (HDF) arrested with respect to division by using 0.5% fetal calf serum in the culture medium are reported. A fraction of cells from irradiated arrested populations, maintained in the arrested state post‐irradiation, is lost from the populations. The extent of cell loss is hence‐dependent and cell strain specific. A Xeroderma pigmentosum cell strain is more sensitive to UV than are normal HDF. No differences in sensitivity were observed when arrested populations established from normal HDF populations of various in vitro ages were used. The length of the pre‐irradiation arrested period affects the sensitivity of normal HDF, which appear more resistant at longer arrested periods, but not the sensitivity of arrested Xeroderma populations. These results suggest that DNA repair processes play a role in maintaining irradiated cells in the arrested state. The suggestion is made that the lethal event caused by UV is an effect on transcription leading to an inhibition of required protein synthesis.