Isolation of rat mammary epithelial cells for polyamine analysis
- 1 November 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 223 (3) , 929-932
- https://doi.org/10.1042/bj2230929
Abstract
Mammary epithelial cells were isolated from either abdominal-inguinal glands or mammary tumors of rats, after enzymic digestion of the tissues, and were analyzed for polyamine content. Optimum conditions were developed for the isolation of cells in sufficient yield for the analysis of polyamines from 1 g of mammary gland or 0.5 g of tumor tissue. Complete recoveries of the polyamines in the tissues were achieved in the isolated epithelial cells.This publication has 20 references indexed in Scilit:
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