Jacalin interacts with Asn‐linked glycopeptides containing multi‐antennary oligosaccharide structure with terminal α‐linked galactose
- 9 January 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 421 (2) , 169-173
- https://doi.org/10.1016/s0014-5793(97)01539-1
Abstract
The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell‐derived d‐[6‐3H]glucosamine‐radiolabeled total glycopeptides containing N‐linked and O‐linked oligosaccharides. The binding of N‐linked glycopeptides to jacalin was abolished by treatment of α‐galactosidase whereas O‐linked glycopeptides were still bound lectin after this treatment. The removal of O‐linked oligosaccharides by mild alkaline/borohydride treatment completely eliminated the lectin binding of α‐galactosidase treated glycopeptides. These results demonstrate that jacalin interacts with cellular glycopeptides containing N‐linked oligosaccharides with terminal α‐galactose residues as well as glycopeptides containing O‐linked oligosaccharides.Keywords
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