RADIATION-DAMAGE TO MOUSE TESTIS CELLS FROM [TC-99M] PERTECHNETATE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 18  (11) , 1116-1122
Abstract
The radiation dose and the biologic damage to mouse testis from i.v. administered 99mTc-pertechnetate were studied. The dose was measured for penetrating and nonpenetrating radiations from 99mTc using calibrated thermoluminescent dosimeters and calculations from the uptake of the nuclide in the testis. The dose was 4.9 rad/mCi. The biologic damage was measured by the decrease in the number of sperm heads in the testis, counted both by hemocytometer and by Coulter counter. In preliminary experiments using external .gamma. radiation from 137Cs, the number of sperm heads reached a minimum 29 days after irradiation. Twenty-nine days after injection of 5.8 mCi of 99mTc, which gave 28 rad to the testis, the number of sperm heads decreased to 70% of control. The biologic effect corresponded to that seen after 40 rad of .gamma.-radiation from 137Cs. The damage to mouse testis cells from internally administered 99mTc, as measured in an in vivo system, appeared at least as significant as that from external .gamma.-irradiation, if not more so.

This publication has 3 references indexed in Scilit: