HLA‐DQB1 AND DQA1 MATCHING BY AMBIENT TEMPERATURE PCR‐SSCP
- 1 December 1995
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 22 (6) , 467-478
- https://doi.org/10.1111/j.1744-313x.1995.tb00283.x
Abstract
We have developed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) protocol for rapid matching of DQA1 and DQB1 alleles. Electrophoresis can be performed at ambient temperature within the range 18-28 degrees C without continuous gel cooling. The method has been tested on 27 patient-potential bone marrow donor pairs for DQB1 and 31 pairs for DQA1. Bone marrow pairs were chosen to represent a broad range of common alleles based upon previous restriction fragment length polymorphism (RFLP) analysis type assignments. Samples were re-typed by PCR with sequence-specific primers (PCR-SSP) and the results compared to matching by PCR-SSCP analysis. There was a 100% correlation between PCR-SSP and PCR-SSCP analysis for DQB1, and a 97% correlation for DQA1 matching.Keywords
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