Comparison of protein composition between schistosomula ofSchistosoma japonicumandS. mansoni

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Abstract
SUMMARY: SDS-PAGE analysis revealed that the protein composition of mechanically transformed schistosomula (ms) of a Japanese strain ofSchistosoma japonicumwas essentially similar to that of a Philippine strain ofS. japonicum. However, the protein components ofS. mansonims were remarkably different from those ofS. japonicum. Specific proteins of Mr60–65.and 30 kDa were found in ms ofS. japonicumandS. mansoni, respectively. In skin-penetrated schistosomula (ss) of the two species, a common protein of 67 kDa was detected. Using two-dimensional electrophoresis, strain-specific polypeptides (9 in a Japanese and 11 in a Philippine strain) were identified in ms ofS. japonicum. Mechanically transformed and skin-penetrated schistosomula ofS. japonicum(Japanese) had, respectively, 7 and 10 specific proteins. Four and 1 specific polypeptides were also detected in two-dimensional profiles of ms and ss ofS. mansoni, respectively.