The in vitro translation of a monocistronic message.

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Abstract
The data presented offer supporting evidence for the earlier conclusion that the approximately 1200 nucleo-tides of STNV-RNA code only for the 400 ammo acid residues of the coat protein. The numerical correspondence and the in vitro data are clearly in agreement with a nonoverlapping triplet code. The relative freedom of the radioautographs from nonspecific peptides indicates that the in vitro system is not introducing significant confusion by incorrect or fragmentary translations. The comparatively accurate translations of a plant virus genome by a bacterial system lends further support for the universality of the translational system. The availability of STNV-RNA as a monocistronic message makes possible a host of informative experiments which can hope to illuminate various aspects of the translational mechanism. These include, among others, the accurate identification of RNA codons and of initiating and terminating sequences of cistrons.

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