Gas Exchange and Phytoluminography of Single Red Kidney Bean Leaves during Periods of Induced Stomatal Oscillations

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Abstract
The capabilities of a new approach to the study of gas exchange and electron transport properties of single, intact leaves were examined. The method combines conventional aspects of analysis with an image intensification system that records the spatial distribution of delayed light emission (DLE) over single leaf surfaces. The combined system was used to investigate physiological perturbations induced by exposure of single leaves of P. vulgaris cv. California Light Red to a combination of SO2 (0.5 .mu.l/l) and ozone (0.1 .mu.l/l). Exposure of one-half of a leaf to this combination induced DLE and stomatal oscillations, but only in the half of the leaf exposed to the combined gases. Examination of phytoluminographs taken during these oscillations revealed distinct leaf patches where the greatest changes in DLE intensity occurred. This phenomenon is evidence that control of stomatal activity of intact plant leaves occurs within discrete leaf areas defined within the vascular network.