Development of Contact Sensitivity to DNFB in Guinea Pigs Genetically Differing in Their Response to DNP Skin Protein Conjugates

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Abstract
Strain 13 guinea pigs sensitized either with dinitrofluorobenzene (DNFB) or with a homologous DNP skin protein conjugate (DNP-GPSP) responded equally well to both an epicutaneous test with the hapten or an intradermal test with the conjugate. Both antigens induced anti-DNP antibodies demonstrated by passive cutaneous anaphylaxis and enhanced DNA synthesis of lymphocytes in vitro. Strain 2 guinea pigs sensitized to DNFB responded only to the epicutaneous test with the hapten but not to the intradermal test with the conjugate, and animals sensitized with DNP-GPSP were unresponsive to both the epicutaneous and the intradermal tests. Anti-DNP antibodies were only induced by DNFB and in no case was DNA synthesis in vitro observed. From these results, the conclusion was drawn that sensitization with a hapten leads to the formation of a set of autologous hapten-protein conjugates and the response to these individual antigens depends on the genetical disposition of the particular strain.

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