AN.IMPROVED METHOD FOR DETERMINATION OF URINARY 17-KETOSTEROIDS*
Open Access
- 1 May 1953
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 13 (5) , 581-586
- https://doi.org/10.1210/jcem-13-5-581
Abstract
In this simple method the final Zimmermann color is extracted with amyl acetate. The definite reduction in titer is comparable to the reduction in titer after micro-Girard separation and after alumina chromatography. Pure 17-ketosteroids and urine extracts assayed by this method show linearity according to Beer''s law.Keywords
This publication has 1 reference indexed in Scilit:
- Die Eliminierung störender Chromogene bei der Bestimmung der 17-Ketosteroide mit m-DinitrobenzolHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1952