Spectrophotometric Determination of Protein in Milk

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Abstract
SUMMARY The spectrophotometric method of Haugaard and Danun (1) for estimating the protein content of skimmilk at wavelength 277 mt~ was modified for raw and pasteurized milk, sterile concentrate, and cream as follows: Add 0.5 ml of butylamine to 0.5 ml of milk, heat to 65 C for 5 rain, add 25 ml of a mixture of 0.3% each of disodium ethylenediamine--tetraaeetate (EDTA) and sodium )auryl sulfate (SLS), add five drops of hydrochloric ttcid and filter. Add one drop of 30% sodium hydroxide solution and read the optical density at 280 m~. For milk changed by the Maillard-type reaction, add 0.5 ml of butyl~mine to 0.5 ml of milk, heat to 65 C and add two drops of sodium borohydride. The remainder of the procedure is the same. Results for whole milk, sterile concentrated milk, and cream agreed with the Kjeldahl method.

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