Eukaryotic Elongation Factor 1A Interacts with the Upstream Pseudoknot Domain in the 3′ Untranslated Region of Tobacco Mosaic Virus RNA
Open Access
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (11) , 5678-5691
- https://doi.org/10.1128/jvi.76.11.5678-5691.2002
Abstract
The genomic RNA of tobacco mosaic virus (TMV), like that of other positive-strand RNA viruses, acts as a template for both translation and replication. The highly structured 3′ untranslated region (UTR) of TMV RNAs plays an important role in both processes; it is not polyadenylated but ends with a tRNA-like structure (TLS) preceded by a conserved upstream pseudoknot domain (UPD). The TLS of tobamoviral RNAs can be specifically aminoacylated and, in this state, can interact with eukaryotic elongation factor 1A (eEF1A)/GTP with high affinity. Using a UV cross-linking assay, we detected another specific binding site for eEF1A/GTP, within the UPDs of TMV and crucifer-infecting tobamovirus (crTMV), that does not require aminoacylation. A mutational analysis revealed that UPD pseudoknot conformation and some conserved primary sequence elements are required for this interaction. Its possible role in the regulation of tobamovirus gene expression and replication is discussed.Keywords
This publication has 83 references indexed in Scilit:
- Role of the 3′-Untranslated Regions of Alfalfa Mosaic Virus RNAs in the Formation of a Transiently Expressed Replicase in Plants and in the Assembly of VirionsJournal of Virology, 2001
- Association between elongation factor-1? and microtubules in vivo is domain dependent and conditionalCell Motility, 2000
- Quantitative Assessment of EF-1α·GTP Binding to Aminoacyl-tRNAs, Aminoacyl-viral RNA, and tRNA Shows Close Correspondence to the RNA Binding Properties of EF-TuJournal of Biological Chemistry, 1999
- Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during InfectionPlant Cell, 1998
- Isolation and Characterization of the 102-Kilodalton RNA-binding Protein That Binds to the 5′ and 3′ Translational Enhancers of Tobacco Mosaic Virus RNAPublished by Elsevier ,1996
- Enhancing effect of the 3′-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitroFEBS Letters, 1994
- Complete nucleotide sequence and genome organization of a tobamovirus infecting cruciferae plantsFEBS Letters, 1994
- RNA pseudoknot domain of tobacco mosaic virus can functionally substitute for a poly(A) tail in plant and animal cells.Genes & Development, 1990
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970