Production of human active BPA from TCGF independent T cell lines that do not excrete HTLV: proof of direct action of MLA‐144 derived BPA using purified BFU‐E

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Abstract
Burst promoting activity (BPA) and colony stimulating activity (CSA) were measured in the supernatants from six T-cell lines which are IL-2 independent and do not excrete human T-cell leukaemia virus. Two cell lines were shown to produce high levels of BPA, namely MLA-144 and the Jurkat cell line. MLA-144 is a gibbon T-cell line which can proliferate and produce BPA in serum free HB101TM medium. The BPA from this line was shown to act directly on BFU-E by studies using highly pruified peripheral blood derived BFU-E. The Jurkat CM had far less activity against purified progenitors. These cell lines will be of value in the study of erythropoietic regulation.