M. tuberculosis genotypic diversity and drug susceptibility pattern in HIV- infected and non-HIV-infected patients in northern Tanzania
Open Access
- 31 May 2007
- journal article
- Published by Springer Nature in BMC Microbiology
- Vol. 7 (1) , 51
- https://doi.org/10.1186/1471-2180-7-51
Abstract
Background: Tuberculosis (TB) is a major health problem and HIV is the major cause of the increase in TB. Sub-Saharan Africa is endemic for both TB and HIV infection. Determination of the prevalence of M. tuberculosis strains and their drug susceptibility is important for TB control. TB positive culture, BAL fluid or sputum samples from 130 patients were collected and genotyped. The spoligotypes were correlated with anti-tuberculous drug susceptibility in HIV-infected and non-HIV patients from Tanzania. Results: One-third of patients were TB/HIV co-infected. Forty-seven spoligotypes were identified. Fourteen isolates (10.8%) had new and unique spoligotypes while 116 isolates (89.2%) belonged to 33 known spoligotypes. The major spoligotypes contained nine clusters: CAS1-Kili 30.0%, LAM11- ZWE 14.6%, ND 9.2%, EAI 6.2%, Beijing 5.4%, T-undefined 4.6%, CAS1-Delhi 3.8%, T1 3.8% and LAM9 3.8%. Twelve (10.8%) of the 111 phenotypically tested strains were resistant to anti-TB drugs. Eight (7.2%) were monoresistant strains: 7 to isoniazid (INH) and one to streptomycin. Four strains (3.5%) were resistant to multiple drugs: one (0.9%) was resistant to INH and streptomycin and the other three (2.7%) were MDR strains: one was resistant to INH, rifampicin and ethambutol and two were resistant to all four anti-TB drugs. Mutation in the kat G gene codon 315 and the rpo B hotspot region showed a low and high sensitivity, respectively, as predictor of phenotypic drug resistance. Conclusion: CAS1-Kili and LAM11-ZWE were the most common families. Strains of the Beijing family and CAS1-Kili were not or least often associated with resistance, respectively. HIV status was not associated with spoligotypes, resistance or previous TB treatment.Keywords
This publication has 37 references indexed in Scilit:
- Molecular Characterization and Drug Resistance Testing of Mycobacterium tuberculosis Isolates from ChadJournal of Clinical Microbiology, 2006
- A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune responseNature, 2004
- Genotypic and Phenotypic Characterization of Drug-Resistant Mycobacterium tuberculosis Isolates from Rural Districts of the Western Cape Province of South AfricaJournal of Clinical Microbiology, 2004
- Characterization of Finnish Mycobacterium tuberculosis Isolates by SpoligotypingJournal of Clinical Microbiology, 2003
- Use of DNA Extracts from Ziehl-Neelsen-Stained Slides for Molecular Detection of Rifampin Resistance and Spoligotyping of Mycobacterium tuberculosisJournal of Clinical Microbiology, 2003
- Mycobacterium tuberculosisBeijing Genotype Strains Associated with Febrile Response to TreatmentEmerging Infectious Diseases, 2001
- Mycobacterium tuberculosis Beijing Genotype Emerging in VietnamEmerging Infectious Diseases, 2000
- The catalase—peroxidase gene and isoniazid resistance of Mycobacterium tuberculosisNature, 1992
- An Outbreak of Tuberculosis with Accelerated Progression among Persons Infected with the Human Immunodeficiency VirusNew England Journal of Medicine, 1992
- Avoiding false positives with PCRNature, 1989