EFFECT OF SERUM FROM PATIENTS WITH CHRONIC RENAL-FAILURE ON ERYTHROID COLONY GROWTH INVITRO

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (3) , 370-375
Abstract
Considerable evidence suggests that insufficient EP [erythropoietin] production and the presence of a toxic factor inhibiting erythropoiesis are 2 major factors responsible for the production of anemia in patients with CRF [chronic renal failure]. The toxic factor can be detected in a number of tissue culture systems. To evaluate its mechanism of action in a proliferation-dependent system, the formation of erythroid colonies in plasma clots containing normal serum and CRF serum was studied using normal mouse marrow cells as the target organ. Fewer colonies were found in cultures containing uremic serum. This effect was greater as the concentration of serum was increased. No differences were found in the size or morphology of colonies formed. Addition of urea and creatinine to normal sera did not affect their ability to support colony growth. Uremic sera had no effect on white cell colony growth in the plasma clot system. Materials inhibitory to erythroid proliferation were present in CRF serum.

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