Immobilization of DNA Hydrogel Plugs in Microfluidic Channels
- 12 February 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 74 (6) , 1436-1441
- https://doi.org/10.1021/ac0156969
Abstract
Acrylamide-modified DNA probes are immobilized in polycarbonate microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions and hybridize with fluorescently tagged complementary DNA. The double-stranded DNA can be chemically denatured, and the chip may be reused with a new analytical sample. Conditions for photopolymerization, hybridization, and denaturation are discussed. We also demonstrate the photopolymerization of plugs containing different DNA probe sequences in one microfluidic channel, thereby enabling the selective detection of multiple DNA targets in one electrophoretic pathway.Keywords
This publication has 18 references indexed in Scilit:
- Binding specificity and stability of duplexes formed by modified oligonucleotides with a 4096-hexanucleotide microarrayNucleic Acids Research, 2001
- DNA Microarrays Based on Noncovalent Oligonucleotide Attachment and Hybridization in Two DimensionsAnalytical Biochemistry, 2001
- Fluidics and sample handling in clinical chemical analysisAnalytica Chimica Acta, 2001
- Novel “Wet Process” Technique Based on Electrochemical Replacement for the Preparation of Fullerene Epitaxial AdlayersLangmuir, 2000
- From Micro- to Nanofabrication with Soft MaterialsScience, 2000
- Micromosaic ImmunoassaysAnalytical Chemistry, 2000
- In situ localized amplification and contact replication of many individual DNA moleculesNucleic Acids Research, 1999
- Microfabricated devices for genetic diagnosticsProceedings of the IEEE, 1998
- UV Laser Machined Polymer Substrates for the Development of Microdiagnostic SystemsAnalytical Chemistry, 1997
- Theoretical analysis of the kinetics of DNA hybridization with gel-immobilized oligonucleotidesBiophysical Journal, 1996