Peroxynitrite‐mediated oxidative protein modifications
- 15 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (3) , 279-282
- https://doi.org/10.1016/0014-5793(95)00307-u
Abstract
Proteins are targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. Using fatty acid-free bovine serum albumin as a model we examined peroxynitrite-mediated protein modifications. The reaction of protein with peroxynitrite resulted in the oxidation of tryptophan and cysteine, in the nitration of tyrosine, in the formation of dityrosine, in the production of 2,4 dinitrophenylhydrazine-reactive carbonyls and in protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species.Keywords
This publication has 20 references indexed in Scilit:
- Quantitation of nitrotyrosine levels in lung sections of patients and animals with acute lung injury.Journal of Clinical Investigation, 1994
- Evidence for nitric oxide‐mediated oxidative damage in chronic inflammation Nitrotyrosine in serum and synovial fluid from rheumatoid patientsFEBS Letters, 1994
- Agonist-Induced Peroxynitrite Production from Endothelial CellsArchives of Biochemistry and Biophysics, 1994
- Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophilsFEBS Letters, 1994
- [23] Oxidative chemistry of peroxynitritePublished by Elsevier ,1994
- Extensive Nitration of Protein Tyrosines in Human Atherosclerosis Detected by ImmunohistochemistryBiological Chemistry Hoppe-Seyler, 1994
- The Reaction of no With SuperoxideFree Radical Research Communications, 1993
- Peroxynitrite formation from macrophage-derived nitric oxideArchives of Biochemistry and Biophysics, 1992
- Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxideChemical Research in Toxicology, 1992
- Peroxynitrite-induced membrane lipid peroxidation: The cytotoxic potential of superoxide and nitric oxideArchives of Biochemistry and Biophysics, 1991