Molecular Cloning of the Rat Metallothionein 1 (MT-1) mRNA Sequence
- 1 January 1983
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 2 (1) , 15-22
- https://doi.org/10.1089/dna.1.1983.2.15
Abstract
Starting with liver mRNA from cadmium chloride-treated rats, we constructed a cDNA library. A mouse metallothionein 1 (MT-1) cDNA restriction fragment (Durnam et al. 1980) was used as a hybridization probe to isolate from this library a cDNA (p2A10) which is a nearly complete copy of the rat MT-1 mRNA sequence. Southern analysis of rat DNA, using p2A10 as a probe, together with the screening of a rat genomic library, reveal that the rat MT-1 gene belongs to a family of related sequences. The rat MT-1 gene is inducible both by glucocorticoids and by metals.This publication has 43 references indexed in Scilit:
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