Overexpression of calbindin‐D 28K in hippocampal progenitor cells increases neuronal differentiation and neurite outgrowth
- 8 November 2005
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 20 (1) , 109-111
- https://doi.org/10.1096/fj.05-4826fje
Abstract
Excitatory stimuli are known to be a potent regulator for induction of neuronal differentiation. Calbindin-D28K buffers intracellular Ca2+ and modifies synaptic functions in neurons. However, the effects of calbindin-D28K on the regulation of activity-induced neuronal differentiation and related biochemical modifications remain unsolved. In the present study, by a gain-of-function study with retroviral vector system and dicer-generated small interfering RNA (d-siRNA) to effectively knock down the expression of calbindin-D28K, we demonstrated that calbindin-D28K at a physiologically relevant level promoted neuronal differentiation and neurite outgrowth. Increase of neuronal differentiation by calbindin-D28K overexpression was concurrent with the expression of basic helix-loop-helix (bHLH) transcriptional factors, phosphorylation of calcium and calmodulin-dependent protein kinase II (CaMKII) and NeuroD at Ser(336). KN-62, a highly specific CaMKII inhibitor, blocked the up-regulation of proneural bHLH genes, p-CaMKII, and pSer(336)NeuroD. Calbindin-D28K appeared to facilitate neuronal differentiation of both fetal and adult hippocampal progenitor cells. Together, these findings establish the novel calbindin-regulated function of CaMKII and NeuroD in control of neuronal differentiation and neurite outgrowth.Keywords
Funding Information
- Manchester Biomedical Research Centre (M103KV010012-03K2201-01230)
- Kemian tekniikan korkeakoulu, Aalto-yliopisto (HY-2002-I, HY-2003-T)
This publication has 28 references indexed in Scilit:
- Retrovirally transduced NCAM140 facilitates neuronal fate choice of hippocampal progenitor cellsJournal of Neurochemistry, 2005
- Calcium Waves Propagate through Radial Glial Cells and Modulate Proliferation in the Developing NeocortexNeuron, 2004
- Overexpression of calbindin D28k in dentate gyrus granule cells alters mossy fiber presynaptic function and impairs hippocampal‐dependent memoryHippocampus, 2004
- Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencingNature Biotechnology, 2003
- Neural cell adhesion molecule (NCAM) promotes the differentiation of hippocampal precursor cells to a neuronal lineage, especially to a glutamatergic neural cell typeExperimental & Molecular Medicine, 2002
- Overexpression of and RNA Interference with the CCAAT Enhancer-Binding Protein on Long-Term Facilitation ofAplysiaSensory to Motor SynapsesLearning & Memory, 2001
- Calcium‐dependent inactivation of high‐threshold calcium currents in human dentate gyrus granule cellsThe Journal of Physiology, 1998
- Stimulation of Ca2+Release-Activated Ca2+Channels as a Potential Mechanism Involved in Non-Genomic 1,25(OH)2-Vitamin D3-Induced Ca2+Entry in Skeletal Muscle CellsBiochemical and Biophysical Research Communications, 1997
- Spontaneous Ca2+ transients in developing hippocampal pyramidal cellsJournal of Neurobiology, 1994
- Evidence for calcium-reducing and excitoprotective roles for the calcium-binding protein calbindin-1328k in cultured hippocampal neuronsNeuron, 1991