Synthesis of cystine peptides 21-25/70-73 and 35-39/56-59 of the β-subunit of human choriogonadotropin
- 12 January 2009
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 35 (2) , 133-140
- https://doi.org/10.1111/j.1399-3011.1990.tb00248.x
Abstract
Syntheses of two asymmetrical cystine peptides with the amino acid residues 21-25/70-73 and 35-39/56-59, based on the linear amino acid sequence and the disulfide bond assignment in the .beta.-subunit of human choriogonadotropin (hCG-.beta.), are described. S-trityl and S-acetamidomethyl peptide fragments of each cystine peptide were prepared in solution phase and were subjected to oxidation with I2/MeOH to form the disulfide bridge. The cystine peptides were characterized by their amino acid analyses and fast atom bombardment mass spectrometry. Immunological characterization by several homologous radioimmunoassay systems showed that peptide 21-25/70-73 had significant hCG, hCG-.beta., and hLH activities while peptide 35-39/56-59 failed to reveal any immunoreactivity.Keywords
This publication has 11 references indexed in Scilit:
- A receptor-binding region in human choriogonadotropin/lutropin beta subunit.Proceedings of the National Academy of Sciences, 1987
- Immunological Properties of the β-Subunit of HumanChorionic Gonadotropin (hCG). II. Properties of a hCGSpecific Antibody Prepared against a Chemical Analog of the β-Subunit*Endocrinology, 1980
- The Synthesis of Cystine Peptides by Iodine Oxidation of S‐Trityl‐cysteine and S‐Acetamidomethyl‐cysteine PeptidesHelvetica Chimica Acta, 1980
- Removal of benzyl-type protecting groups from peptides by catalytic transfer hydrogenation with formic acidThe Journal of Organic Chemistry, 1979
- Location of major antigenic sites of the β-subunit of human chorionic gonadotropinBiochemistry, 1978
- Immunological properties of the β-subunit of human chorionic gonadotropinBiochemical and Biophysical Research Communications, 1976
- Zur selektiven acidolytischen Abspaltbarkeit der tert.‐Butyloxycarbonyl‐GruppeEuropean Journal of Organic Chemistry, 1971
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963