Specific Protein Analysis by Light-Scatter Measurement with a Miniature Centrifugal Fast Analyzer

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Abstract
A miniature Centrifugal Fast Analyzer has been modified for fluorescence and light-scatter measurements by using several rotors developed for this purpose. The modified system has been used to evaluate the feasibility of adapting specific protein analyses, such as IgG, IgA, IgM, C'3 complement component, and α-1-antitrypsin, to the Centrifugal Fast Analyzer. A study of reaction conditions has revealed that the addition of polyethylene glycol 6000 (Carbowax) to the dilution medium increases the rate of reaction and allows apparent equilibrium to be achieved in less than 60 s. Furthermore, scatter intensity is enhanced. This system can be used to make rapid immunoglobulin measurements with only microliter volumes of antibody (2-7 µl), without the need of sample blanks. Determination of antigen excess by a method that involves dynamic injection of antibody is also discussed.

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