Gliadin uptake in human enterocytes. Differences between coeliac patients in remission and control individuals.
Open Access
- 1 November 1992
- Vol. 33 (11) , 1487-1492
- https://doi.org/10.1136/gut.33.11.1487
Abstract
The pepsin trypsin digest of the wheat prolamin gliadin (PT-gliadin) is deleterious to the small intestinal mucosa of coeliac patients. The handling of PT-gliadin by the intestinal epithelium in coeliac patients in remission and control individuals was investigated by in vivo instillation of PT-gliadin. The uptake of PT-gliadin was monitored by immunofluorescence microscopy of intestinal biopsy specimens, using affinity purified PT-gliadin antibodies. Control individuals show weak staining in the apical region of the enterocytes thereby showing an uptake of PT-gliadin. Coeliac patients have a conspicuous fluorescence in relation to the lateral membrane/intercellular space of enterocytes and intense staining intracellularly in the apical region. There is only weak staining in the enterocytes after the instillation was terminated, indicating an intracellular clearance. The study shows that normal enterocytes are able to take up PT-gliadin. The increased uptake in coeliac patients might be of importance for the pathogenesis either by direct toxicity or by presentation to immunocompetent cells. Furthermore, the results are in agreement with the suggestion of a functional alteration in the zonula occludens in the intestinal epithelium of coeliac patients.Keywords
This publication has 20 references indexed in Scilit:
- Patients with coeliac disease have a characteristic gliadin antibody patternClinica Chimica Acta; International Journal of Clinical Chemistry, 1986
- Acute gluten challenge in treated adult coeliac disease: a morphometric and enzymatic study.Gut, 1985
- Studies of Intestinal Lymphoid Tissue: IX. Dose-Dependent, Gluten-Induced Lymphoid Infiltration of Coeliac Jejunal EpitheliumScandinavian Journal of Gastroenterology, 1985
- Breakdown of gliadin peptides by intestinal brush borders from coeliac patients.Gut, 1984
- A PERSISTENT DEFECT IN INTESTINAL PERMEABILITY IN COELIAC DISEASE DEMONSTRATED BY A 51Cr-LABELLED EDTA ABSORPTION TESTThe Lancet, 1983
- Further evidence of a primary mucosal defect in coeliac disease: In vitro mucosal digestion studies in coeliac patients in remission, their relatives, and control subjectsGut, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Protein a from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulinsFEBS Letters, 1972
- Studies of jejunal mucosal digestion of peptic-tryptic digests of wheat protein in coeliac disease.Gut, 1966