Association between HLA Determinants and Complement Receptors on Human Lymphocytes

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Abstract
The relationship between the major histocompatibility complex (MHC) and functional receptors on the surface of human lymphocytes was studied. HLA antisera were tested for their effect on the formation of E[erythrocyte]-, EA[E, antibody]-, and EAC[E,A, complement]- rosettes by human peripheral blood lymphocytes (PBL). Antisera to various HLA specificities inhibited the formation of EAC-rosettes, but had no effect on the formation of E-rosettes. The formation of EA-rosettes was inhibited by HLA antisera only in part among the individuals tested. Anti-.beta.2-microglobulin serum resembled HLA antisera in its effect on the formation of the various rosettes. HLA determinants and C receptors are different entities on the cell surface since elimination of C receptors by trypsin treatment does seem to affect the expression of HLA antigens on the cell surface. EAC'' receptors are apparently located close to HLA determinants.