Characteristics of Baculovirus Preparations Processed from Living and Dead Larvae2

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Abstract
Preparations of the nucleopolyhedrosis virus of Heliothis spp.,Baculovirus heliothis, processed from dead larvae produced more polyhedral inclusion bodies per larva than preparations processed from living larvae. Also, virus harvested from dead larvae was consistently more active than virus harvested from living larvae. Although bacterial counts of preparations from dead larvae were higher than counts from living larvae, they were still below the microbiological standard permitted for nonfat dry milk. Preparations processed by lyophilization or acetone extraction and immediately bioassayed were similar in activity to that of freshly prepared aqueous suspensions of diseased larvae.