Generic method for on‐line extraction of drug substances in the presence of biological matrices using turbulent flow chromatography
- 28 January 2002
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (5) , 421-426
- https://doi.org/10.1002/rcm.593
Abstract
The use of liquid chromatography/mass spectrometry (LC/MS) to quantify drugs in biological matrices has been well established over the last decade. Extremely fast LC/MS methods are commonplace in the pharmaceutical industry for high-throughput Absorption, Distribution, Metabolism and Excretion (ADME) screening. However, to truly take full advantage of high-throughput ADME screening, a generic method is needed that eliminates the need to develop a new method for each new compound being screened. New developments in the stationary phase of turbulent flow columns has allowed us to develop an on-line biological sample cleanup method that is suitable for over 99% of the compounds in the Cephalon database. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
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