Degradation of bacterial cell walls by immobilized lysozyme
- 1 December 1988
- journal article
- research article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 19 (3) , 243-249
- https://doi.org/10.1007/bf02921496
Abstract
Lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. The enzyme was bound as a Schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to controlled pore aminoglass via pyridino-4-aldehyde and BrCN. The latter preparations had activities up to 2.5% of the free lysozyme.Keywords
This publication has 12 references indexed in Scilit:
- Surface charge measurements on Micrococcus lysodeikticus and the catalytic implications for lysozymeBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Polyacrylhydrazido-agarose: Preparation via periodate oxidation and use for enzyme immobilization and affinity chromatographyJournal of Chromatography A, 1981
- Nonporous magnetic supports for proteases, cell-lytic enzymes, and ribonuclease: Limits of reactant sizeBiotechnology & Bioengineering, 1979
- Catalytic implications of electrostatic potentials: The lytic activity of lysozyme as a modelJournal of Molecular Biology, 1976
- Optimization of Activities of Immobilized Lysozyme, α-Chymotrypsin, and LipasePublished by Springer Nature ,1974
- Lysis ofMicrococcus lysodeikticus by lysozyme covalently immobilized on cellulose and polyacrylamideBiotechnology & Bioengineering, 1973
- Synthesis of a Matrix-supported Enzyme in Non-aqueous ConditionsNature, 1973
- The measurement of lysozyme activity and the ultra-violet inactivation of lysozymeBiochimica et Biophysica Acta, 1952
- BUFFERS OF PH-2 TO PH-12 FOR USE IN ELECTROPHORESIS1950