Two groups of phosphatidylethanolamine in Vibrio sp. strain ABE-1 separated by silica gel thin-layer chromatography.

Publisher Website
Google Scholar
Abstract
We analyzed a previously unknown phospholipid (PL-2) in the psychrophilic bacterium, Vibrio ABE-1. In thin-layer chromatography, it moved close to phsophatidylethanolamine but had a different Rf value. The polar head group of PL-2 was ethanolamine, and the sn-glycerol carbon atoms. This demonstrates that PL-2 is a species of phosphatidyl-bond/ethanolamine was 1 : 1. 1:2.0:1.1. The chloroform- and water-soluble fractions after treatment with phospholipase C were 1,2-diacylglycerol and phosphoryl ethanolamine, respectively. The phsophatidylethanolamine of this bacterium has mainly C16 fatty acids especially hexadecenoic acid (16 : 1). PL-2 has higher amounts of saturated fatty acids such as hexadecanoic acid (16:0) and short chain fatty acids with less than 15 carbon atoms. This demonstrates that PL-2 is a species of phosphatidylethanolamine which has fatty acids that are different in average chain length and degree of unsaturation. The possible role of PL-2 in the phase separation of membrane phospholipid is discussed.

This publication has 0 references indexed in Scilit: